Safety Data Sheet - Chloroform

SECTION 1: Identification of the substance/mixture and of the company/undertaking

Chemical Name Chloroform

Product Form Substance

Catalogue No. AL1299

CAS Number 67-66-3

EC Number 200-663-8

EC Index Number 602-006-00-4

Grade For Molecular Biology

HSN Code 29031300

Molecular Formula CHCl3

SECTION 1: Identification of the substance/mixture and of the company/undertaking

1.1 Product identifier

Chemical Name Chloroform

Catalogue No. AL1299

Product form Substance

CAS Number 67-66-3

EC Number 200-663-8

Grade For Molecular Biology

EC Index Number 602-006-00-4

Chemical Formula CHCl3

HSN Code 29031300

Synonyms Trichloromethane, Methyl trichloride

Chemical Structure -

1.2 Relevant identified uses of the substance or mixture and uses advised against

Use of the substance/mixture Solvent for organic compounds, used in nucleic acid extraction.

Uses advised against Not specified

1.3 Details of the supplier of the safety data sheet

Company identification Address:- 102, B wing, Savgan Heights, RTO Lane,Andheri (W), Mumbai-400 053Maharashtra, India.

1.4 Emergency Contact Details

Phone / e-Mail Phone : +91 22 26317055 Mobile : +91 9820385757 e-mail : info@alphachemika.co.in

SECTION 2: Hazards identification

2.1 Classification of the substance or mixture

Classification according to Regulation (EC) No 1272/2008 Acute Tox. 4; Skin Irrit. 2; Eye Irrit. 2; Acute Tox. 3; Carc. 2; Repr. 2; STOT RE 1; STOT RE 2

Classification source ECHA C&L Inventory via PubChem CID 6212 - hazard classes notified by at least 50% of companies; pictograms and signal word derived from those statements

2.2 Label elements

Hazard pictograms Acute Toxic, Health Hazard

Signal word Danger

Hazard statements H302: Harmful if swallowed; H315: Causes skin irritation; H319: Causes serious eye irritation; H331: Toxic if inhaled; H351: Suspected of causing cancer; H361: Suspected of damaging fertility or the unborn child; H372: Causes damage to organs through prolonged or repeated exposure; H373: May causes damage to organs through prolonged or repeated exposure

2.3 Other hazards

Other hazards Suspected carcinogen, may cause reproductive toxicity.

SECTION 3: Composition/information on ingredients

3.1 Substance

Name Chloroform

CAS No. 67-66-3

SECTION 4: First aid measures

4.1 Description of first aid measures

First-aid measures after inhalation If this chemical has been inhaled, remove the exposed person to fresh air at once. If breathing has stopped, perform artificial respiration. Keep the person warm and at rest.

First-aid measures after skin contact If this chemical contacts the skin, promptly wash the contaminated skin with soap and water. If this chemical penetrates the clothing, promptly remove the clothing and wash the skin with soap and water. Get medical attention promptly.

First-aid measures after eye contact If this chemical contacts the eyes, immediately wash (irrigate) the eyes with large amounts of water, occasionally lifting the lower and upper lids. Get medical attention immediately.

First-aid measures after ingestion If this chemical has been swallowed, get medical attention immediately.

4.2 Most important symptoms and effects, both acute and delayed

Symptoms relating to use Headache, dizziness, nausea, respiratory distress.

4.3 Indication of any immediate medical attention and special treatment needed

General information Treat symptomatically. Show this safety data sheet to the treating physician.

SECTION 5: Fire-fighting measures

5.1 Extinguishing media

Suitable extinguishing media Use water spray, alcohol-resistant foam, dry chemical or carbon dioxide. Advice for firefighters: Wear self-contained breathing apparatus for firefighting if necessary.

Unsuitable extinguishing media Not specified

5.2 Special hazards arising from the substance or mixture

Special procedures The vapour is heavier than air and may accumulate in lowered spaces causing a deficiency of oxygen.

5.3 Advice for firefighters

Protection during firefighting Do not enter fire area without proper protective equipment, including respiratory protection.

SECTION 6: Accidental release measures

6.1.1 For non-emergency personnel

Emergency procedures Evacuate area. Ventilate and contain spill. Use appropriate protective equipment.

6.3 Methods and material for containment and cleaning up

Methods for cleaning up Evacuate danger area! Consult an expert! Personal protection: complete protective clothing including self-contained breathing apparatus. Do NOT let this chemical enter the environment. Collect leaking liquid in sealable containers. Absorb remaining liquid in sand or inert absorbent.

6.4 Reference to other sections

Reference Not specified

SECTION 7: Handling and storage

7.1 Precautions for safe handling

Precautions for safe handling Use in a fume hood. Wear appropriate personal protective equipment (PPE).

Hygiene measures Wash hands and other exposed areas with mild soap and water before eating, drinking or smoking and when leaving work.

7.2 Conditions for safe storage, including any incompatibilities

Storage conditions Store only in original container. Separated from food and feedstuffs and incompatible materials. See Chemical Dangers. Ventilation along the floor. Store in an area without drain or sewer access.

SECTION 8: Exposure controls/personal protection

8.2 Exposure controls

Hand protection Protective gloves (e.g. nitrile rubber)

Eye protection Chemical safety goggles or safety glasses

Skin and body protection Wear suitable protective clothing

Respiratory protection Use a NIOSH-approved respirator if ventilation is inadequate.

SECTION 9: Physical and chemical properties

9.1 Information on basic physical and chemical properties

Physical state Liquid

Colour Colorless

Odour Pleasant, etheric, nonirritating

Odour threshold Not established

pH value Not applicable

Relative evaporation rate (butylacetate=1) 0.97

Melting point -63.47 °C

Freezing point -63.5 °C

Boiling point 61.12 °C

Flash point 11.7 °C

Auto-ignition temperature 550 °C

Decomposition temperature Not established

Flammability (solid, gas) Not flammable

Vapour pressure VP: 100 mm Hg at 10.4 °C

Relative vapour density at 20°C 4.12 (Air = 1)

Density 1.4788 g/cu cm at 25 °C

Solubility In water, 7.95X10+3 mg/L at 25 °C

Log Pow 1.97

Viscosity, kinematic 0.56 cSt

Viscosity, dynamic 0.84 mPa·s

Explosive properties Not explosive

Oxidising properties Not oxidizing

Explosive limits Not applicable

SECTION 10: Stability and reactivity

10.1 Reactivity

Reactivity Stable under normal conditions. Reacts with strong oxidizing agents.

10.2 Chemical stability

Chemical stability Decomposes at ordinary temp in sunlight in the absence of air, and in the dark in the presence of air.

10.3 Possibility of hazardous reactions

Possibility of hazardous reactions Decomposes on heating. This produces toxic and corrosive fumes of hydrogen chloride (see ICSC 0163), phosgene (see ICSC 0007) and chlorine (see ICSC 0126). Reacts violently with strong bases, strong oxidants, alkalis, acetone and some metals such as aluminium, magnesium and zinc.

10.4 Conditions to avoid

Conditions to avoid Avoid contact with incompatible materials and excessive heat.

10.5 Incompatible materials

Incompatible materials Strong oxidizers, strong bases, and amines.

10.6 Hazardous decomposition products

Hazardous decomposition products When heated to decomposition forms phosgene gas.

SECTION 11: Toxicological information

11.1 Information on toxicological effects

Acute toxicity LD50 Rat oral 2180 mg/kg; LD50 Rabbit oral 9827 mg/kg; LD50 Rat intragastric 2000 mg/kg

Skin corrosion/irritation H315: Causes skin irritation

Serious eye damage/irritation H319: Causes serious eye irritation

Respiratory or skin sensitisation Not classified (based on available data)

Germ cell mutagenicity Not classified (based on available data)

Carcinogenicity H351: Suspected of causing cancer

Specific target organ toxicity (single exposure) Not classified (based on available data)

Specific target organ toxicity (repeated exposure) H372: Causes damage to organs through prolonged or repeated exposure; H373: May causes damage to organs through prolonged or repeated exposure

SECTION 12: Ecological information

12.1 Toxicity

Toxicity information Not specified

12.2 Persistence - degradability

Persistence - degradability Not specified

12.3 Bioaccumulative potential

Bioaccumulative potential Not specified

12.4 Mobility in soil

Mobility in soil Not specified

12.6 Other adverse effects

Environmental precautions Not specified

SECTION 13: Disposal considerations

13.1 Waste treatment methods

Additional Info Dispose of contents/container in accordance with local, regional, national and international regulations.

SECTION 14: Transport information

14.1 UN number

UN-No. (ADR) 1888

UN-No. (IMDG) 1888

UN-No. (IATA) 1888

UN-No. (ADN) 1888

UN-No. (RID) 1888

14.2 UN proper shipping name

Proper Shipping Name (ADR) Chloroform

Proper Shipping Name (IMDG) Chloroform

Proper Shipping Name (IATA) Chloroform

Proper Shipping Name (ADN) Chloroform

Proper Shipping Name (RID) Chloroform

14.3 Transport hazard class(es)

Transport hazard class(es) 6.1

14.4 Packing group

Packing group (ADR) III

Packing group (IMDG) III

Packing group (IATA) III

Packing group (ADN) III

Packing group (RID) III

14.5 Environmental hazards

Dangerous for the environment Not specified

Marine pollutant Not specified

Other information No supplementary information available

SECTION 15: Regulatory information

15.1 Safety, health and environmental regulations/legislation specific for the substance or mixture

15.1.1 EU-Regulations Not specified

SECTION 16: Other Information

Disclaimer The information provided in this Safety Data Sheet is correct to the best of our knowledge, information and belief at the date of its publication. The information is designed only as a guidance for safe handling, use, processing, storage, transportation, disposal and release and is not to be considered a guarantee or quality specification. The information relates only to the specific material designated and may not be valid for such material used in combination with any other materials or in any process, unless specified in the text.