Safety Data Sheet - Ethidium bromide

SECTION 1: Identification of the substance/mixture and of the company/undertaking

Chemical Name Ethidium bromide

Product Form Substance

Catalogue No. AL2033

CAS Number 1239-45-8

EC Number 214-984-6

EC Index Number 611-007-00-2

Grade AR grade

HSN Code 29339900

Molecular Formula C21H20BrN3

SECTION 1: Identification of the substance/mixture and of the company/undertaking

1.1 Product identifier

Chemical Name Ethidium bromide

Catalogue No. AL2033

Product form Substance

CAS Number 1239-45-8

EC Number 214-984-6

Grade AR grade

EC Index Number 611-007-00-2

Chemical Formula C21H20BrN3

HSN Code 29339900

Synonyms Ethidium bromide, 1,3-bis(4-(dimethylamino)phenyl)isoquinolinium bromide

Chemical Structure -

1.2 Relevant identified uses of the substance or mixture and uses advised against

Use of the substance/mixture Laboratory reagent for DNA/RNA visualization

Uses advised against Not specified

1.3 Details of the supplier of the safety data sheet

Company identification Address:- 102, B wing, Savgan Heights, RTO Lane,Andheri (W), Mumbai-400 053Maharashtra, India.

1.4 Emergency Contact Details

Phone / e-Mail Phone : +91 22 26317055 Mobile : +91 9820385757 e-mail : info@alphachemika.co.in

SECTION 2: Hazards identification

2.1 Classification of the substance or mixture

Classification according to Regulation (EC) No 1272/2008 Muta. 2; Acute Tox. 2 *; Acute Tox. 4 *

Classification source CLP Annex VI via PubChem CID 14710

2.2 Label elements

Hazard pictograms Acute Toxic, Health Hazard

Signal word Danger

Hazard statements H302: Harmful if swallowed; H330: Fatal if inhaled; H341: Suspected of causing genetic defects

Precautionary statements P203, P260, P264, P270, P271, P280, P284, P301+P317, P304+P340, P316, P318, P320, P330, P403+P233, P405, P501

2.3 Other hazards

Other hazards Mutagenic and suspected carcinogen; handle with care.

SECTION 3: Composition/information on ingredients

3.1 Substance

Name Ethidium bromide

CAS No. 1239-45-8

SECTION 4: First aid measures

4.1 Description of first aid measures

First-aid measures after inhalation Move to fresh air. If symptoms persist, seek medical attention.

First-aid measures after skin contact Wash with soap and water. Seek medical attention if irritation develops.

First-aid measures after eye contact Rinse cautiously with water for several minutes. Remove contact lenses if present and easy to do. Continue rinsing and seek medical attention.

First-aid measures after ingestion Do not induce vomiting. Rinse mouth and seek medical attention immediately.

4.2 Most important symptoms and effects, both acute and delayed

Symptoms relating to use Symptoms of exposure may include irritation of the respiratory tract, skin, and eyes; nausea; and potential mutagenic effects.

4.3 Indication of any immediate medical attention and special treatment needed

General information Treat symptomatically. Show this safety data sheet to the treating physician.

SECTION 5: Fire-fighting measures

5.1 Extinguishing media

Suitable extinguishing media Water spray, carbon dioxide, dry chemical powder, foam.

Unsuitable extinguishing media Not specified

5.2 Special hazards arising from the substance or mixture

Special procedures Toxic fumes may be released in a fire. Use water spray to cool containers.

5.3 Advice for firefighters

Protection during firefighting Do not enter fire area without proper protective equipment, including respiratory protection.

SECTION 6: Accidental release measures

6.1.1 For non-emergency personnel

Emergency procedures Evacuate area and ventilate. Wear appropriate protective equipment. Contain spill and collect for disposal.

6.3 Methods and material for containment and cleaning up

Methods for cleaning up Sweep spilled substance into sealable containers. Then wash away with plenty of water.

6.4 Reference to other sections

Reference Not specified

SECTION 7: Handling and storage

7.1 Precautions for safe handling

Precautions for safe handling Avoid contact with skin and eyes. Use appropriate personal protective equipment (PPE) including gloves and goggles.

Hygiene measures Wash hands and other exposed areas with mild soap and water before eating, drinking or smoking and when leaving work.

7.2 Conditions for safe storage, including any incompatibilities

Storage conditions Fireproof. Separated from strong oxidants and food and feedstuffs.

SECTION 8: Exposure controls/personal protection

8.2 Exposure controls

Hand protection Protective gloves (e.g. nitrile rubber)

Eye protection Chemical safety goggles or safety glasses

Skin and body protection Wear suitable protective clothing

Respiratory protection Use a NIOSH-approved respirator if exposure limits are exceeded or if irritation occurs.

SECTION 9: Physical and chemical properties

9.1 Information on basic physical and chemical properties

Physical state Solid

Colour Dark red-purple

Odour Odourless

Odour threshold No data available

pH value No data available

Relative evaporation rate (butylacetate=1) No data available

Melting point 260-262 °C (decomposes)

Freezing point No data available

Boiling point 250 °C

Flash point >100 °C

Auto-ignition temperature No data available

Decomposition temperature No data available

Flammability (solid, gas) No

Vapour pressure 1.2X10-12 mm Hg at 25 °C

Relative vapour density at 20°C No data available

Density 0.34 g/cm³

Solubility Solubility in 2-methoxyethanol is 20 mgmL; ethanol, 2 mgmL

Log Pow log Kow= -0.38

Viscosity, kinematic No data available

Viscosity, dynamic No data available

Explosive properties Not explosive

Oxidising properties Not oxidizing

Explosive limits No data available

SECTION 10: Stability and reactivity

10.1 Reactivity

Reactivity Stable under recommended storage conditions. Avoid strong oxidizing agents.

10.2 Chemical stability

Chemical stability Stable under recommended storage conditions.

10.3 Possibility of hazardous reactions

Possibility of hazardous reactions Decomposes on heating. This produces toxic gases including hydrogen bromide (see ICSC 0282) and nitrogen oxides (see ICSCs 0930 and 1311). Reacts violently with strong oxidants.

10.4 Conditions to avoid

Conditions to avoid Avoid contact with incompatible materials and excessive heat.

10.5 Incompatible materials

Incompatible materials Strong oxidizing agents, strong acids, and bases.

10.6 Hazardous decomposition products

Hazardous decomposition products When heated to decomposition it emits very toxic fumes of hydrogen bromide and nitrogen oxides.

SECTION 11: Toxicological information

11.1 Information on toxicological effects

Acute toxicity LD50 Mouse subcutaneous 110 mg/kg; LD50 Mouse intraperitoneal 20 mg/kg; LD50 Rat iv 27 mg/kg

Skin corrosion/irritation Not classified (based on available data)

Serious eye damage/irritation Not classified (based on available data)

Respiratory or skin sensitisation Not classified (based on available data)

Germ cell mutagenicity H341: Suspected of causing genetic defects

Carcinogenicity Not classified (based on available data)

Specific target organ toxicity (single exposure) Not classified (based on available data)

Specific target organ toxicity (repeated exposure) Not classified (based on available data)

SECTION 12: Ecological information

12.1 Toxicity

Toxicity information Not specified

12.2 Persistence - degradability

Persistence - degradability Not specified

12.3 Bioaccumulative potential

Bioaccumulative potential Not specified

12.4 Mobility in soil

Mobility in soil Not specified

12.6 Other adverse effects

Environmental precautions Not specified

SECTION 13: Disposal considerations

13.1 Waste treatment methods

Additional Info Dispose of contents/container in accordance with local, regional, national and international regulations.

SECTION 14: Transport information

14.1 UN number

UN-No. (ADR) 2811

UN-No. (IMDG) 2811

UN-No. (IATA) 2811

UN-No. (ADN) 2811

UN-No. (RID) 2811

14.2 UN proper shipping name

Proper Shipping Name (ADR) Toxic solid, organic, n.o.s. (Ethidium bromide)

Proper Shipping Name (IMDG) Toxic solid, organic, n.o.s. (Ethidium bromide)

Proper Shipping Name (IATA) Toxic solid, organic, n.o.s. (Ethidium bromide)

Proper Shipping Name (ADN) Toxic solid, organic, n.o.s. (Ethidium bromide)

Proper Shipping Name (RID) Toxic solid, organic, n.o.s. (Ethidium bromide)

14.3 Transport hazard class(es)

Transport hazard class(es) 6.1

14.4 Packing group

Packing group (ADR) I

Packing group (IMDG) I

Packing group (IATA) I

Packing group (ADN) I

Packing group (RID) I

14.5 Environmental hazards

Dangerous for the environment Not specified

Marine pollutant Not specified

Other information No supplementary information available

SECTION 15: Regulatory information

15.1 Safety, health and environmental regulations/legislation specific for the substance or mixture

15.1.1 EU-Regulations Not specified

SECTION 16: Other Information

Disclaimer The information provided in this Safety Data Sheet is correct to the best of our knowledge, information and belief at the date of its publication. The information is designed only as a guidance for safe handling, use, processing, storage, transportation, disposal and release and is not to be considered a guarantee or quality specification. The information relates only to the specific material designated and may not be valid for such material used in combination with any other materials or in any process, unless specified in the text.