Auramine (M.S.)
- CAS Number
- 2465-27-2
- HSN Code
- 32041321
- Molecular Formula
- C17H22N3Cl
- Catalogue Number
- AL0511
- Purity
- Min. 85%
- Molecular Weight
- 303.84 g/mol
Complies with : IP | BP | USP | EP | ACS
ISO 9001
ISO 14001
GMP
COA with every batch
Key Properties
Auramine O is a hydrochloride obtained by combining 4,4'-carbonimidoylbis(N,N-dimethylaniline) with one molar equivalent of hydrogen chloride. A fluorescent stain for demonstrating acid fast organisms in a method similar to the Ziehl Neelsen.
Also known as Auramine O, Basic Yellow 2.
- Catalogue No.
- AL0511
- CAS Number
- 2465-27-2
- Molecular Formula
- C17H22N3Cl
- Molecular Weight
- 303.84 g/mol
- HSN Code
- 32041321
- Grade
- C.I. 41000
- Product Form
- Substance
Melting point: >250 °C (dec); Solubility: 10 mgml in water; 60 mgml in ethylene glycol methyl ether; 20 mg/ml in EtOH.
- Appearance
- Yellow powder
- Shelf Life
- -
- Packaging
- Available in: 25 gm glass bottle, 100 gm glass bottle
- Storage
- PRECAUTIONS FOR "CARCINOGENS": Storage site should be as close as practical to lab in which carcinogens are to be used, so that only small quantities required for expt need to be carried.
1.1 — Product identifier
- Chemical Name
- Auramine (M.S.)
- Catalogue No.
- AL0511
- Product form
- Substance
- CAS Number
- 2465-27-2
- EC Number
- 219-567-2
- Grade
- C.I. 41000
- EC Index Number
- Not specified
- Chemical Formula
- C17H22N3Cl
- HSN Code
- 32041321
- Synonyms
- Auramine O, Basic Yellow 2
1.2 — Relevant identified uses of the substance or mixture and uses advised against
- Use of the substance/mixture
- Fluorescent stain for microbiological applications
- Uses advised against
- Not specified
1.3 — Details of the supplier of the safety data sheet
- Company identification
- Address:- 102, B wing, Savgan Heights, RTO Lane,Andheri (W), Mumbai-400 053Maharashtra, India.
1.4 — Emergency Contact Details
- Phone / e-Mail
- Phone : +91 22 26317055 Mobile : +91 9820385757 e-mail : info@alphachemika.co.in
2.1 — Classification of the substance or mixture
- Classification according to Regulation (EC) No 1272/2008
- Acute Tox. 4; Acute Tox. 3; Carc. 2
- Classification source
- ECHA C&L Inventory via PubChem CID 17170 - hazard classes notified by at least 50% of companies; pictograms and signal word derived from those statements
2.2 — Label elements
- Hazard pictograms
- Acute Toxic, Health Hazard
- Signal word
- Danger
- Hazard statements
- H302: Harmful if swallowed; H311: Toxic in contact with skin; H351: Suspected of causing cancer
2.3 — Other hazards
- Other hazards
- Suspected carcinogen; may cause respiratory irritation.
3.1 — Substance
- Name
- Auramine (M.S.)
- CAS No.
- 2465-27-2
4.1 — Description of first aid measures
- First-aid measures after inhalation
- Move to fresh air. If symptoms persist, seek medical attention.
- First-aid measures after skin contact
- IMMEDIATELY flood affected skin with water while removing and isolating all contaminated clothing. Gently wash all affected skin areas thoroughly with soap and water.
- First-aid measures after eye contact
- First check the victim for contact lenses and remove if present. Flush victim's eyes with water or normal saline solution for 20 to 30 minutes while simultaneously calling a hospital or poison control center.
- First-aid measures after ingestion
- Rinse mouth. Do not induce vomiting. Seek medical attention immediately.
4.2 — Most important symptoms and effects, both acute and delayed
- Symptoms relating to use
- Symptoms of exposure may include respiratory irritation, skin irritation, and eye irritation.
4.3 — Indication of any immediate medical attention and special treatment needed
- General information
- Treat symptomatically. Show this safety data sheet to the treating physician.
5.1 — Extinguishing media
- Suitable extinguishing media
- Water spray, carbon dioxide, dry chemical powder, foam.
- Unsuitable extinguishing media
- Not specified
5.2 — Special hazards arising from the substance or mixture
- Special procedures
- May emit toxic fumes in a fire. Use water spray to cool containers.
5.3 — Advice for firefighters
- Protection during firefighting
- Do not enter fire area without proper protective equipment, including respiratory protection.
6.1.1 — For non-emergency personnel
- Emergency procedures
- Evacuate area and ventilate. Wear appropriate protective equipment.
6.3 — Methods and material for containment and cleaning up
- Methods for cleaning up
- Collect spillage with suitable absorbent material and dispose of in accordance with local regulations.
6.4 — Reference to other sections
- Reference
- Not specified
7.1 — Precautions for safe handling
- Precautions for safe handling
- Avoid contact with skin and eyes. Use in a well-ventilated area and wear appropriate protective equipment.
- Hygiene measures
- Wash hands and other exposed areas with mild soap and water before eating, drinking or smoking and when leaving work.
7.2 — Conditions for safe storage, including any incompatibilities
- Storage conditions
- PRECAUTIONS FOR "CARCINOGENS": Storage site should be as close as practical to lab in which carcinogens are to be used, so that only small quantities required for expt need to be carried.
8.2 — Exposure controls
- Hand protection
- Protective gloves (e.g. nitrile rubber)
- Eye protection
- Chemical safety goggles or safety glasses
- Skin and body protection
- Wear suitable protective clothing
- Respiratory protection
- Use appropriate respiratory protection if dust is generated.
9.1 — Information on basic physical and chemical properties
- Physical state
- Solid
- Colour
- Yellow-orange to brown
- Odour
- Odourless
- Odour threshold
- Not specified
- pH value
- Not specified
- Relative evaporation rate (butylacetate=1)
- Not specified
- Melting point
- >250 °C (dec)
- Freezing point
- Not specified
- Boiling point
- Not specified
- Flash point
- Not specified
- Auto-ignition temperature
- Not specified
- Decomposition temperature
- Not specified
- Flammability (solid, gas)
- Not specified
- Vapour pressure
- Not specified
- Relative vapour density at 20°C
- Not specified
- Density
- Not specified
- Solubility
- 10 mgml in water; 60 mgml in ethylene glycol methyl ether; 20 mg/ml in EtOH
- Log Pow
- Not specified
- Viscosity, kinematic
- Not specified
- Viscosity, dynamic
- Not specified
- Explosive properties
- Not explosive
- Oxidising properties
- Not oxidizing
- Explosive limits
- Not specified
10.1 — Reactivity
- Reactivity
- Stable under normal conditions. Avoid strong oxidizing agents.
10.2 — Chemical stability
- Chemical stability
- Stable under recommended storage conditions.
10.3 — Possibility of hazardous reactions
- Possibility of hazardous reactions
- No dangerous reactions known under normal conditions of use.
10.4 — Conditions to avoid
- Conditions to avoid
- Avoid contact with incompatible materials and excessive heat.
10.5 — Incompatible materials
- Incompatible materials
- Strong oxidizing agents.
10.6 — Hazardous decomposition products
- Hazardous decomposition products
- Not specified
11.1 — Information on toxicological effects
- Acute toxicity
- Toxic if swallowed, inhaled, or absorbed through skin.
- Skin corrosion/irritation
- Not classified (based on available data)
- Serious eye damage/irritation
- Not classified (based on available data)
- Respiratory or skin sensitisation
- Not classified (based on available data)
- Germ cell mutagenicity
- Not classified (based on available data)
- Carcinogenicity
- H351: Suspected of causing cancer
- Specific target organ toxicity (single exposure)
- Not classified (based on available data)
- Specific target organ toxicity (repeated exposure)
- Not classified (based on available data)
12.1 — Toxicity
- Toxicity information
- Not specified
12.2 — Persistence - degradability
- Persistence - degradability
- Not specified
12.3 — Bioaccumulative potential
- Bioaccumulative potential
- Not specified
12.4 — Mobility in soil
- Mobility in soil
- Not specified
12.6 — Other adverse effects
- Environmental precautions
- Not specified
13.1 — Waste treatment methods
- Additional Info
- Dispose of contents/container in accordance with local, regional, national and international regulations.
14.1 — UN number
- UN-No. (ADR)
- UN 2811
- UN-No. (IMDG)
- UN 2811
- UN-No. (IATA)
- UN 2811
- UN-No. (ADN)
- UN 2811
- UN-No. (RID)
- UN 2811
14.2 — UN proper shipping name
- Proper Shipping Name (ADR)
- Toxic solid, organic, n.o.s.
- Proper Shipping Name (IMDG)
- Toxic solid, organic, n.o.s.
- Proper Shipping Name (IATA)
- Toxic solid, organic, n.o.s.
- Proper Shipping Name (ADN)
- Toxic solid, organic, n.o.s.
- Proper Shipping Name (RID)
- Toxic solid, organic, n.o.s.
14.3 — Transport hazard class(es)
- Transport hazard class(es)
- 6.1
14.4 — Packing group
- Packing group (ADR)
- III
- Packing group (IMDG)
- III
- Packing group (IATA)
- III
- Packing group (ADN)
- III
- Packing group (RID)
- III
14.5 — Environmental hazards
- Dangerous for the environment
- Not specified
- Marine pollutant
- Not specified
- Other information
- No supplementary information available
15.1 — Safety, health and environmental regulations/legislation specific for the substance or mixture
- 15.1.1 EU-Regulations
- Not specified
Auramine is used as a fluorescent stain in clinical microbiology, particularly for the auramine-rhodamine acid-fast stain method to detect Mycobacterium tuberculosis.
ISO 9001
ISO 14001
GMP
COA with every batch
- Batch Number
- AL-2026-AL00192
- Manufacturing Date
- September-2026
- Expiry Date
- September-2031
- Molecular Weight
- 303.84 g/mol
| Test | Standard | Observed |
|---|---|---|
| Description | Yellow colored powder | Yellow colored powder |
| Absorption | Max. 431-433nm | 432 nm |
| Absorptivity ( A1%; 1cm=432 nm , pH 6.0 on dried substance) | 880 - 940 | 931.6 |
| Loss on drying ( 105°C ) | < 2% | 1.2% |
| Suitability for microscopy | Passes test | Passes test |
The above product complies with LR grade
Technical Data Sheet
| Grade | Assay/Purity | Melting/Boiling Point | Moisture/LOD | Residue on Ignition | Related Substances | Solubility |
|---|---|---|---|---|---|---|
| C.I. 41000 | Min. 85% | m.p. >250 °C (dec) | Max. 5% | Max. 1% | Not specified | 10 mgml in water; 60 mgml in ethylene glycol methyl ether; 20 mg/ml in EtOH |
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