Ethidium bromide
- CAS Number
- 1239-45-8
- HSN Code
- 29339900
- Molecular Formula
- C21H20BrN3
- Catalogue Number
- AL2033
- Purity
- ≥ 98%
- Molecular Weight
- 394.31 g/mol
Complies with : IP | BP | USP | EP | ACS
ISO 9001
ISO 14001
GMP
COA with every batch
Key Properties
Ethidium bromide is the organic bromide salt of ethidium. It has a role as a geroprotector, an intercalator and a trypanocidal drug.
Also known as Ethidium bromide, 1,3-bis(4-(dimethylamino)phenyl)isoquinolinium bromide.
- Catalogue No.
- AL2033
- CAS Number
- 1239-45-8
- Molecular Formula
- C21H20BrN3
- Molecular Weight
- 394.31 g/mol
- HSN Code
- 29339900
- Grade
- AR grade
- Product Form
- Substance
Melting point: 260-262 °C (decomposes); Flash point: >100 °C; Density: 0.34 g/cm³; Solubility: Solubility in 2-methoxyethanol is 20 mgmL; ethanol, 2 mgmL.
- Appearance
- Dark red crystals from alcohol
- Shelf Life
- 2 years
- Packaging
- Available in: 1 gm glass bottle, 5 gm glass bottle, 25 gm glass bottle
- Storage
- Fireproof. Separated from strong oxidants and food and feedstuffs.
1.1 — Product identifier
- Chemical Name
- Ethidium bromide
- Catalogue No.
- AL2033
- Product form
- Substance
- CAS Number
- 1239-45-8
- EC Number
- 214-984-6
- Grade
- AR grade
- EC Index Number
- 611-007-00-2
- Chemical Formula
- C21H20BrN3
- HSN Code
- 29339900
- Synonyms
- Ethidium bromide, 1,3-bis(4-(dimethylamino)phenyl)isoquinolinium bromide
1.2 — Relevant identified uses of the substance or mixture and uses advised against
- Use of the substance/mixture
- Laboratory reagent for DNA/RNA visualization
- Uses advised against
- Not specified
1.3 — Details of the supplier of the safety data sheet
- Company identification
- Address:- 102, B wing, Savgan Heights, RTO Lane,Andheri (W), Mumbai-400 053Maharashtra, India.
1.4 — Emergency Contact Details
- Phone / e-Mail
- Phone : +91 22 26317055 Mobile : +91 9820385757 e-mail : info@alphachemika.co.in
2.1 — Classification of the substance or mixture
- Classification according to Regulation (EC) No 1272/2008
- Muta. 2; Acute Tox. 2 *; Acute Tox. 4 *
- Classification source
- CLP Annex VI via PubChem CID 14710
2.2 — Label elements
- Hazard pictograms
- Acute Toxic, Health Hazard
- Signal word
- Danger
- Hazard statements
- H302: Harmful if swallowed; H330: Fatal if inhaled; H341: Suspected of causing genetic defects
- Precautionary statements
- P203, P260, P264, P270, P271, P280, P284, P301+P317, P304+P340, P316, P318, P320, P330, P403+P233, P405, P501
2.3 — Other hazards
- Other hazards
- Mutagenic and suspected carcinogen; handle with care.
3.1 — Substance
- Name
- Ethidium bromide
- CAS No.
- 1239-45-8
4.1 — Description of first aid measures
- First-aid measures after inhalation
- Move to fresh air. If symptoms persist, seek medical attention.
- First-aid measures after skin contact
- Wash with soap and water. Seek medical attention if irritation develops.
- First-aid measures after eye contact
- Rinse cautiously with water for several minutes. Remove contact lenses if present and easy to do. Continue rinsing and seek medical attention.
- First-aid measures after ingestion
- Do not induce vomiting. Rinse mouth and seek medical attention immediately.
4.2 — Most important symptoms and effects, both acute and delayed
- Symptoms relating to use
- Symptoms of exposure may include irritation of the respiratory tract, skin, and eyes; nausea; and potential mutagenic effects.
4.3 — Indication of any immediate medical attention and special treatment needed
- General information
- Treat symptomatically. Show this safety data sheet to the treating physician.
5.1 — Extinguishing media
- Suitable extinguishing media
- Water spray, carbon dioxide, dry chemical powder, foam.
- Unsuitable extinguishing media
- Not specified
5.2 — Special hazards arising from the substance or mixture
- Special procedures
- Toxic fumes may be released in a fire. Use water spray to cool containers.
5.3 — Advice for firefighters
- Protection during firefighting
- Do not enter fire area without proper protective equipment, including respiratory protection.
6.1.1 — For non-emergency personnel
- Emergency procedures
- Evacuate area and ventilate. Wear appropriate protective equipment. Contain spill and collect for disposal.
6.3 — Methods and material for containment and cleaning up
- Methods for cleaning up
- Sweep spilled substance into sealable containers. Then wash away with plenty of water.
6.4 — Reference to other sections
- Reference
- Not specified
7.1 — Precautions for safe handling
- Precautions for safe handling
- Avoid contact with skin and eyes. Use appropriate personal protective equipment (PPE) including gloves and goggles.
- Hygiene measures
- Wash hands and other exposed areas with mild soap and water before eating, drinking or smoking and when leaving work.
7.2 — Conditions for safe storage, including any incompatibilities
- Storage conditions
- Fireproof. Separated from strong oxidants and food and feedstuffs.
8.2 — Exposure controls
- Hand protection
- Protective gloves (e.g. nitrile rubber)
- Eye protection
- Chemical safety goggles or safety glasses
- Skin and body protection
- Wear suitable protective clothing
- Respiratory protection
- Use a NIOSH-approved respirator if exposure limits are exceeded or if irritation occurs.
9.1 — Information on basic physical and chemical properties
- Physical state
- Solid
- Colour
- Dark red-purple
- Odour
- Odourless
- Odour threshold
- No data available
- pH value
- No data available
- Relative evaporation rate (butylacetate=1)
- No data available
- Melting point
- 260-262 °C (decomposes)
- Freezing point
- No data available
- Boiling point
- 250 °C
- Flash point
- >100 °C
- Auto-ignition temperature
- No data available
- Decomposition temperature
- No data available
- Flammability (solid, gas)
- No
- Vapour pressure
- 1.2X10-12 mm Hg at 25 °C
- Relative vapour density at 20°C
- No data available
- Density
- 0.34 g/cm³
- Solubility
- Solubility in 2-methoxyethanol is 20 mgmL; ethanol, 2 mgmL
- Log Pow
- log Kow= -0.38
- Viscosity, kinematic
- No data available
- Viscosity, dynamic
- No data available
- Explosive properties
- Not explosive
- Oxidising properties
- Not oxidizing
- Explosive limits
- No data available
10.1 — Reactivity
- Reactivity
- Stable under recommended storage conditions. Avoid strong oxidizing agents.
10.2 — Chemical stability
- Chemical stability
- Stable under recommended storage conditions.
10.3 — Possibility of hazardous reactions
- Possibility of hazardous reactions
- Decomposes on heating. This produces toxic gases including hydrogen bromide (see ICSC 0282) and nitrogen oxides (see ICSCs 0930 and 1311). Reacts violently with strong oxidants.
10.4 — Conditions to avoid
- Conditions to avoid
- Avoid contact with incompatible materials and excessive heat.
10.5 — Incompatible materials
- Incompatible materials
- Strong oxidizing agents, strong acids, and bases.
10.6 — Hazardous decomposition products
- Hazardous decomposition products
- When heated to decomposition it emits very toxic fumes of hydrogen bromide and nitrogen oxides.
11.1 — Information on toxicological effects
- Acute toxicity
- LD50 Mouse subcutaneous 110 mg/kg; LD50 Mouse intraperitoneal 20 mg/kg; LD50 Rat iv 27 mg/kg
- Skin corrosion/irritation
- Not classified (based on available data)
- Serious eye damage/irritation
- Not classified (based on available data)
- Respiratory or skin sensitisation
- Not classified (based on available data)
- Germ cell mutagenicity
- H341: Suspected of causing genetic defects
- Carcinogenicity
- Not classified (based on available data)
- Specific target organ toxicity (single exposure)
- Not classified (based on available data)
- Specific target organ toxicity (repeated exposure)
- Not classified (based on available data)
12.1 — Toxicity
- Toxicity information
- Not specified
12.2 — Persistence - degradability
- Persistence - degradability
- Not specified
12.3 — Bioaccumulative potential
- Bioaccumulative potential
- Not specified
12.4 — Mobility in soil
- Mobility in soil
- Not specified
12.6 — Other adverse effects
- Environmental precautions
- Not specified
13.1 — Waste treatment methods
- Additional Info
- Dispose of contents/container in accordance with local, regional, national and international regulations.
14.1 — UN number
- UN-No. (ADR)
- 2811
- UN-No. (IMDG)
- 2811
- UN-No. (IATA)
- 2811
- UN-No. (ADN)
- 2811
- UN-No. (RID)
- 2811
14.2 — UN proper shipping name
- Proper Shipping Name (ADR)
- Toxic solid, organic, n.o.s. (Ethidium bromide)
- Proper Shipping Name (IMDG)
- Toxic solid, organic, n.o.s. (Ethidium bromide)
- Proper Shipping Name (IATA)
- Toxic solid, organic, n.o.s. (Ethidium bromide)
- Proper Shipping Name (ADN)
- Toxic solid, organic, n.o.s. (Ethidium bromide)
- Proper Shipping Name (RID)
- Toxic solid, organic, n.o.s. (Ethidium bromide)
14.3 — Transport hazard class(es)
- Transport hazard class(es)
- 6.1
14.4 — Packing group
- Packing group (ADR)
- I
- Packing group (IMDG)
- I
- Packing group (IATA)
- I
- Packing group (ADN)
- I
- Packing group (RID)
- I
14.5 — Environmental hazards
- Dangerous for the environment
- Not specified
- Marine pollutant
- Not specified
- Other information
- No supplementary information available
15.1 — Safety, health and environmental regulations/legislation specific for the substance or mixture
- 15.1.1 EU-Regulations
- Not specified
Used as the classic intercalating fluorescent stain for visualizing DNA/RNA bands in agarose gel electrophoresis. It is also utilized in various molecular biology applications, including nucleic acid quantification and detection.
ISO 9001
ISO 14001
GMP
COA with every batch
- Batch Number
- AL-2026-AL0675B
- Manufacturing Date
- September-2026
- Expiry Date
- September-2031
- Molecular Weight
- 394.31 g/mol
| Test | Standard | Observed |
|---|---|---|
| Description | Dark red crystalline powder | Dark red crystalline powder |
| Assay | Min. 98.0% | 98.74% |
| Absorption maximum (In Methanol) | 524-527 nm | 527 nm |
| Absorptivity (A1%/1 cm, in Methanol on dried substance) | 155-165 | 158 |
| Loss on drying (130°C/4 hours) | < 7 % |
The above product complies with AR grade
Technical Data Sheet
| Grade | Assay/Purity | Melting/Boiling Point | Moisture/LOD | Residue on Ignition | Related Substances | Solubility |
|---|---|---|---|---|---|---|
| AR grade | ≥ 98% | m.p. 260-262 °C (decomposes) | ≤ 0.5% | ≤ 0.1% | None detected | Solubility in 2-methoxyethanol is 20 mgmL; ethanol, 2 mgmL |
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